Kell phenotyping using Gel
A colleague wonders if anyone is doing Kell phenotype testing in Gel? A manufacturer sells Gel cards for E and C phenotyping, but not for Kell. The inquiring colleague has thought about trying to use Gel cards for Kell typing and is curious if anyone else has tried this, and if so, with what sort of validation plan and test results.
The following comments have been submitted in response.
ADDENDA Sept. 23, 2011
- A Blood Bank Supervisor reports that twelve years ago they were told by their Ortho Clinical Diagnostics trainer when they started using gel that they could use any antisera in gel that is labeled as "Indirect Antiglobin test". If the antisera is for "slide, tube or microplate" it cannot be used in gel. The responding colleagues lab screens for K, S, FYA and FYB in gel. They report doing over 100 parallel tests for each antigen without any questionable results.
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